논문검색
Author Seong Man Kim, Seong Jin Cho, Woo Young Jang, Duck Hwan Kim, Hyung Sik Shin, Myoung Kuk Jang, Hak Yang Kim, Eun Sook Nam
Place of duty 1Laboratory of Gastroenterology and Hepatology, Departments of 2Pathology, and 3Internal Medicine, Hallym University Medical Center, Seoul, Korea.
Title Expression of Maspin is associated with the Intestinal Type of Gastric Adenocarcinoma
Publicationinfo Cancer Res Treat. 2005 Aug; 037(04): 228-232.
Key_word Maspin,Gastric adenocarcinoma,Nested RT-PCR,Immunohistochemistry
Full-Text
Abstract Purpose: Maspin is known as a tumor suppressor gene, but its significance has been questioned in various human cancers. The aim of this study was to investigate the expression pattern of Maspin in human gastric adenocarcinomas and its possible correlation with clinicopathological findings. Materials and Methods: The expression of Maspin mRNA was measured by nested RT-PCR using 60 frozen adenocarcinomas of the stomach and 31 noncancerous tissues from the proximal resection margin. Immunohistochemical study for Maspin protein expression was carried out using 62 paraffin-embedded tissues, composed of both cancer and noncancerous tissues. Results: Maspin mRNA expression was detected in 80.0% (48 of 60) of the gastric adenocarcinomas, but in only 22.6% (7 of 31) of the normal gastric mucosa (pp<0.001). The positive rate of Maspin protein expression was higher in the adenocarcinomas than the normal tissues (62.9% vs. 27.4%, p<0.05). In addition, the intestinal type of tumors showed significantly higher expression levels compared to the diffuse type of tumors (81.5% vs. 48.6%, pp<0.05). Conclusion: Our results suggest that Maspin is frequently expressed in human gastric cancers, and its expression might be associated with tumorigenesis of the intestinal type of gastric cancer. (Cancer Res Treat. 2005; 37:228-232)